Peptide Injection Guide

This guide is designed to help you access all the resources you need to draw up and inject your peptide medications.

Injection Instructions

Click the drop down for details on each step of safely injecting your peptide medication.

Clean & Prepare Your Space

Wash your hands and sanitize your space.

Gather Materials

Materials Needed

Reconstituted Peptide(s)

DETAILS

Your peptide needs to be in liquid form prior to injection. If your peptide is still in powder form, reconstitute it prior to moving on.

Reconstitution Instructions

Injection Syringe

DETAILS

Injection syringes are included with every peptide order and can be found in your injection kit. 

Alcohol Wipes

DETAILS

Alcohol wipes are included with every peptide order and can be found in your injection kit. 

Dosing Instructions

DETAILS

Ensure you know what your peptide dose in units is for each peptide you will be injecting. Dosing instructions can be found using the link below, or by referring to the specific dose recommended by your provider.

Dosing Instructions

Select Your Injection Site

Inject your peptides into subcutaneous fat.

Common subcutaneous injection sites include:

Upper Outer Arms

Lower Abdomen  (avoiding the area 2 inches around the navel)

Upper Glutes

Upper Outer Thighs

*You can inject BPC + TB4 close to an injury site for more localized effects. 

Ensure You Know The Dose For Each Peptide You Are Injecting In Units

Standard Dosing Instructions

The table below outlines that standard dosing guidelines for each injectable peptide. Follow these instructions unless otherwise specified by your provider. 

Detailed Dosing Instructions

 PEPTIDE

  units / DOSE

  mg / DOSE

  TIME OF DAY

  FREQUENCY

  CYCLE

Sermorelin

20 units

0.25mg

Evening

5x weekly

3 months on / 1 off

CJC/Ipamorelin

20 units

0.25mg

Evening

5x weekly

3 months on / 1 off

Tesamorelin

20 units

1.25mg

Morning

5x weekly

3 months on / 1 off

BPC-157

20 units

0.5mg

Any Time

5x weekly

3 months on / 1 off

TB4 Thymosin Beta 4

20 units

0.5mg

Any Time

5x weekly

3 months on / 1 off

BPC + TB4

20 units

0.5/0.5mg

Any Time

5x weekly

3 months on / 1 off

GHK-Cu

20 units

2mg

Any Time

5x weekly

3 months on / 1 off

Pentosan

50 units

125mg

Any Time

2x weekly

As Directed

Selank

20 units

0.5mg

Morning

5x weekly

3 months on / 1 off

Epitalon

20 units

1mg

Evening

5x weekly

As Directed

Pinealon

20 units

1mg

Evening

5x weekly

3 months on / 1 off

Melanotan 2

20 units

0.5mg

Evening

3x weekly

As Directed 

NAD+

50 units

50mg

Morning

2x weekly

Continuously 

SS31

50 units

5mg

Morning

2x weekly

3 months on / 1 off

MOTS-c

50 units

5mg

Morning

2x weekly

3 months on / 1 off

TA1 Thymosin Alpha 1

20 units

1mg

Any Time

5x weekly

As Directed

VIP

20 units

1mg

Morning

5x weekly

As Directed 

Tirzepatide

25 units

2.5mg

Any Time

1x weekly

Continuously

Semaglutide

12.5 units

0.25mg

Any Time

1x weekly

Continuously

Retatrutide

20 units

2mg

Any Time

1x weekly

Continuously

Performance Stack

60 units

Multi-dose

Morning

4x weekly

Cycle with other stacks

Longevity Stack

60 units

Multi-dose

Morning

4x weekly

Cycle with other stacks

Mitochondrial Stack

60 units

Multi-dose

Morning

4x weekly

Cycle with other stacks

Draw Up Your Peptide Dose (Video Instructions)
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Draw Up Your Peptide Dose

Once you know the units of the dose you are drawing up:

1.  Wipe the rubber tops of all peptide vials with an alcohol wipe. Allow them to air dry completely.

2. Uncap your syringe and insert the needle into the first peptide vial.

3. Turn the vial upside down, ensuring the needle tip is below the liquid level.

4. Slowly pull back the plunger to draw desired amount of peptide. (Refer to the unit value of your dose).

5. Remove the needle from the vial without touching the needle to any surface.

Inject Your Peptides (Video Instructions)

Inject Your Peptides

1. Wipe the chosen injection site with an alcohol wipe. Allow to air dry.

2. Pinch a small section of your skin to create a fold.

3. Insert your syringe at a slight angle, ensuring you are getting into the subcutaneous fat.

4. Slowly push in the plunger to inject the peptides.

5. Once all medication is injected, carefully withdraw the syringe, and safely dispose of the needle (see below).
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Properly Dispose Of Your Syringe

 Properly Dispose of Your Syringe
  • Place the used syringe in a sharps container if accessible.
  • If not, you can re-cap your syringes and place them in a safe container. You can dispose of your container of used syringes by returning it to our office at your next appointment or peptide pick up, so we can put it in our sharps container.
  • You can also bring your used syringes to a local pharmacy or other sharps container near you.

Other Important Peptide Topics

Click the drop down for details on each topic.

Storage

Powdered Peptides

Store your powdered peptides in the freezer or refrigerator. Wait to reconstitute them until you are ready to use the vial.

SHELF LIFE: When stored in a refrigerator or freezer, lyophilized peptides can remain stable for up to two years. They can also be stored at room temperature for several weeks with only minimal degradation. 

Reconstituted Peptides

Once reconstituted, peptides should be stored in the refrigerator to preserve their effectiveness. 

SHELF LIFE: Reconstituted peptides can remain stable for up to 7 days without refrigeration, but it is best to keep them consistently cold to maintain their maximum potency. Typically, reconstituted peptides are stable for 3-6 months if stored properly in the refrigerator, and with good handling, they may remain effective for longer.

Nasal Sprays, Creams

Nasal Sprays & Cream peptides should be stored in the refrigerator to preserve their effectiveness. 

SHELF LIFE: Nasal Sprays have a shelf life of 3 months after opening, up to 6 months when stored properly in the fridge. Creams have a shelf life of 6 months after opening. Both last up to 12 months if kept in the freezer .

Injection Reaction Guide

Extension Health Injection Reaction Guide: WHY THEY HAPPEN & HOW TO REDUCE THEM

If you’ve ever experienced redness, itching, swelling, or even small hives after injecting peptides like CJC-1295, Ipamorelin, or Tesamorelin, you’re not alone. These types of skin reactions are fairly common, but that doesn’t mean you have to tolerate them without support. In this guide, we’ll walk you through why these reactions occur and what you can do to minimize or prevent them, helping you stay comfortable and consistent with your peptide protocol.

Injection Reaction Guide pdf

If Taking Multiple Peptides, Decide If & Which Peptides You Will Be Injecting In The Same Syringe

Some peptides can be mixed one syringe to reduce the number of injections, but others might lose their effectiveness or cause unwanted reactions. Use the Mixing Chart to help guide your use.

 

Assess reaction separately first, before mixing. 

If you do mix peptides in a single syringe, it is important to inject them within a few minutes after drawing them up.

Peptide Mixing Chart

Balancing The Pressure Inside Your Peptide Vials

How to Balance the Pressure When Reconstituting a Peptide Vial

When a vial is reconstituted, the pressure inside should be balanced. If your peptide was reconstituted by our staff, the pressure has already been adjusted before dispensing. However, if you are reconstituting your own peptide, you must balance the pressure immediately after adding the reconstitution solution.

Follow the reconstitution instructions carefully. When you reach the step where you have added the reconstitution solution (at 49 seconds on the video tutorial):

1. Before removing the syringe – Gently lift it so the needle is above the liquid but still inside the vial under the rubber stopper.

2. Allow the stopper to adjust – Release the plunger and let the vial equalize the pressure naturally. The stopper should rise to match the amount of liquid originally added.

3. Carefully remove the syringe – Put the cap back on and either dispose of the syringe or save it for reconstituting future vials.
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How to Check the Pressure in the Vial Before Drawing Up Your Peptide

Proper pressure balance in your vial is essential for an easy and accurate draw of your peptide dose. This is especially important when drawing multiple peptides into the same syringe, as unequal pressure can make the process more difficult.

1. Prepare your syringe – Fill it halfway with air.

2. Insert the syringe into the vial – Do not push or pull the plunger; allow the stopper to naturally adjust.

3. Observe the pressure balance – The plunger should not be forced in or out. If the stopper adjusts on its own, the pressure is balanced.

4. Remove the syringe – You can now proceed to draw up your peptide dose as instructed.
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Drawing Up Multiple Peptides Into The Same Syringe

Instructions for Combining Peptides

1. BALANCE THE PRESSURE INSIDE OF THE PEPTIDE VIAL PRIOR TO DRAWING UP.

2. Draw up your first peptide dose as usual.

3. Do not inject yet. Keep the syringe with the first peptide.

4. Insert this syringe into the second vial. Draw up the next peptide’s required dosing into the same syringe.

5. Repeat for additional peptides. Continue adding peptides until you have all your doses combined in one syringe.

 

Example:
If you are prescribed 20 units each of BPC-157, TB4, and GHK-Cu, your syringe will have a total of 60 units. This way, you only need one injection instead of three.

 

Tips:

1. Inject your mixed peptides within a few minutes of drawing them up. Do not preload the syringes and let them sit.

2. Ensure the peptides in your syringe are clear and not cloudy before injection.

Reconstitution Guide

Materials Needed

10mL Syringe

Reconstitution Solution

Alcohol Wipes

Peptide Vial(s)

Reconstitution Chart

Reconstitution Instructions
  1. Prepare your workspace – Clean your area and wash your hands thoroughly.
  2. Prepare the vials – Remove the caps from both the peptide vial and the reconstitution solution vial.
  3. Sanitize – Wipe the tops of both vials with an alcohol wipe.
  4. Fill the syringe – Remove the cap from the syringe and insert the needle into the bacteriostatic water vial.
  5. Draw the solution – Turn the vial upside down and slowly draw up the required amount of bacteriostatic water based on the Reconstitution Chart.
  6. Inject into the peptide vial – Insert the needle at a slight angle to minimize bubbling and slowly push the plunger to add the liquid.
  7. Balance the pressure – Before removing the syringe, lift it so the needle is above the liquid but still inside the vial. Release the plunger to allow the vial to equalize pressure.
  8. Remove the syringe carefully – Cap the syringe and dispose of it or save it for future use.
  9. Dissolve the peptide – Gently swirl the vial until the powder is fully dissolved. Do not shake the vial.
  10. Store properly – Place the reconstituted peptide in the refrigerator.
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